Journal: Biomolecules

Article Title: Secretome from Uterine Cervical Mesenchymal Stem Cells as a Protector of Neuronal Cells Against Oxidative Stress and Inflammation

doi: 10.3390/biom15101402

Figure Lengend Snippet: Flow cytometric analysis of hUCESC. Representative figure of hUCESC labeled with FITC- and PE- antibodies and analyzed by flow cytometry. Histograms show the expression of surface antigens.

Article Snippet: In order to characterize the isolated hUCESC, the cells were stained with a panel of specific monoclonal antibodies for mesenchymal stem cells: CD31-PE, CD34-PE, CD44-PE, CD45-FITC, CD73-PE (Becton Dickinson, Biosciences Pharmingen, San Diego, CA, USA), CD90-FITC, and CD105-FITC (Miltenyi Biotec, Bergisch Gladbach, Germany).

Techniques: Labeling, Flow Cytometry, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The 3D World of Spheroids: Searching for an Optimal Method of Fabricating Pro-Reparative Cardiospheres

doi: 10.3390/ijms262412025

Figure Lengend Snippet: Cell and extracellular matrix (ECM) composition of mCSs formed in ULA U-well plates and PDL-coated plates. ( a , b ): Images of mCSs stained by endothelial cell (EC) marker (CD31, red), progenitor cell markers (c-Kit, red; Sca-1, red; Oct4, red), smooth-muscle cell marker (SMA, green), mesenchymal markers (CD105, red; CD90, green; CD73, green), and ECM proteins (COL I, green; FN, green). Nuclei were stained with DAPI. Yellow arrows indicate positive staining. ( c ) Bar plot of a positive staining cell fraction per nuclei within the mCS slice, n = 3, * p < 0.05. ( d ) Bar plot of fluorescence intensity signal normalized per mCS slice area, n = 3, ** p < 0.01. SMA—smooth muscle actin, COL I—collagen type I, FN—fibronectin.

Article Snippet: The EDCs were trypsinized, washed in ice-cold FASC buffer (5% FBS, 0.1% sodium azide in Ca 2+ , Mg 2+ -free DPBS), and incubated with primary antibodies to CD105 (#120404, Biolegend, San Diego, CA, USA), Sca-1 conjugated with PE/Cy7 (E-AB-F1191UH, Elabscience, Wuhan, China), CD90 conjugated with FITC (E-AB-F1283UC, Elabscience), CD31 (#553370; BD, San Diego, CA, USA), and CD45 conjugated with PE (E-AB-F1136UD, Elabscience) or with isotype IgGs for 30 min on ice.

Techniques: Staining, Marker, Fluorescence

Journal: International Journal of Molecular Sciences

Article Title: The 3D World of Spheroids: Searching for an Optimal Method of Fabricating Pro-Reparative Cardiospheres

doi: 10.3390/ijms262412025

Figure Lengend Snippet: Cell and extracellular matrix (ECM) composition of mCSs formed in ULA U-well plates and PDL-coated plates. ( a , b ): Images of mCSs stained by endothelial cell (EC) marker (CD31, red), progenitor cell markers (c-Kit, red; Sca-1, red; Oct4, red), smooth-muscle cell marker (SMA, green), mesenchymal markers (CD105, red; CD90, green; CD73, green), and ECM proteins (COL I, green; FN, green). Nuclei were stained with DAPI. Yellow arrows indicate positive staining. ( c ) Bar plot of a positive staining cell fraction per nuclei within the mCS slice, n = 3, * p < 0.05. ( d ) Bar plot of fluorescence intensity signal normalized per mCS slice area, n = 3, ** p < 0.01. SMA—smooth muscle actin, COL I—collagen type I, FN—fibronectin.

Article Snippet: The primary antibodies include antibodies against CD31 (#553370, BD), c-Kit (MAB1356; R&D Systems, Minneapolis, MN, USA), Sca-1 (#108101, Biolegend, San Diego, CA, USA), Oct4 (ab18976, Abcam, Cambridge, UK), αSMA (ab32575; Abcam), CD105 (#105801, Biolegend), CD90 (E-AB-F1283UC, Elabscience), CD73 (sc-25603, Santa Cruz Biotechnology, Dallas, TX, USA), collagen I (A1352; Abclonal, Wuhan, China), and fibronectin (PAA037Mu01; Cloud-Clone Corp., Wuhan, China).

Techniques: Staining, Marker, Fluorescence

Journal: Cell Transplantation

Article Title: Adipose-derived stem cells inhibit dendritic cell migration by secreting tumor necrosis factor-α-stimulated gene 6 to improve the allogeneic skin transplantation survival rate in mice

doi: 10.1177/09636897251376125

Figure Lengend Snippet: Characteristics of adipose-derived stem cells. (a) Positive expression for CD90 and CD105 and negative expression for CD19 and CD34. (b), (c) Differentiation of ADSCs into adipocytes and osteoblasts. After adipogenic differentiation, newly differentiated adipocytes had lipid droplets identified by Oil Red O staining. Osteogenic differentiation was confirmed by Alizarin Red S staining. ADSCs: adipose-derived stem cells; CD: cluster of differentiation.

Article Snippet: Passage 3 cells were stained with antibodies against CD90 (E-AB-F1283C) (Elabscience, Wuhan, China), CD105 (E-AB-F1233UE) (Elabscience, Wuhan, China), CD19 (E-AB-F1004E) (Elabscience, Wuhan, China), and CD34 (E-AB-F1284D) (Elabscience, Wuhan, China).

Techniques: Derivative Assay, Expressing, Staining

Journal: Biomaterials Research

Article Title: External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force

doi: 10.34133/bmr.0265

Figure Lengend Snippet: ECM coating using the layer-by-layer (LbL) technique was deposited on the cell surface. (A) Gelatin-RBITC/HyA-FITC-coated hMSCs via FACS analysis were shifted to the positive region (Gelatin-RBITC in red fluorescence and HyA–FITC in green fluorescence). (B) Mean fluorescence intensity (MFI) of the ECM coating layer on hMSC was changed with deposition of increasing numbers of layers based on FACS analysis ( n = 4).

Article Snippet: 153037, Mouse anti Human CD90: FITC Bio-Rad Formerly AbD Serotec), and CD105 (endoglin, Lot.

Techniques: Fluorescence

Journal: Biomaterials Research

Article Title: External-Force-Offset Effects of ECM Coating Layers on hMSCs Subjected to External Physical Force

doi: 10.34133/bmr.0265

Figure Lengend Snippet: Surface morphological changes in bare hMSCs and ECM-hMSCs. (A) Confocal laser scanning microscopy (CLSM) images of Gelatin-RBITC (red)/HyA-FITC (green)-coated hMSCs. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) in blue (magnification: ×400, scale bar: 20 μm). (B) The cell diameter of bare hMSCs and ECM-hMSCs was calculated using ImageJ based on the images of CLSM ( n = 50). (C) Scanning electron microscope (SEM) image depicting the cell surface morphology between the bare hMSCs and ECM-hMSCs independent experiments (inner image—magnification: ×5.0 k, scale bar: 10 μm; outer image—magnification: ×10.0 k, scale bar: 5 μm).

Article Snippet: 153037, Mouse anti Human CD90: FITC Bio-Rad Formerly AbD Serotec), and CD105 (endoglin, Lot.

Techniques: Confocal Laser Scanning Microscopy, Staining, Microscopy